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Fig. S5 Nhsl1b is not required for neuroepithelial apico-basal polarity. (A-D) Confocal images of cross sections at the level of rhombomere (r)4 showing normal apico-basal polarity in the neuroepithelium of 24 hours post-fertilization (hpf) wild-type (A,C) and nhsl1bfh131 mutant (B,D) embryos. ZO-1 marks sub-apical tight junctions of progenitors (A,B) and γ-tubulin marks apical centrosomes (C,D). isl1:GFP marks FBM neurons which accumulate in r4 in nhsl1bfh131 mutants (B,D). Scale bar: 50 µm.
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