Fig. S7
Effect of Dram1 on autophagosomal vesicles and fusion events. (A) Representative confocal micrograph of GFP-Lc3 embryos transiently expressing mCherry- Dram1. Co-localization is indicated with arrowheads.
(B-D) Transmission electron micrograph of control or dram1 RNA injected embryos infected with Mm. (B) Bacteria (indicated by an arrowhead) inside an autophagolysome, as characterized by the presence of cytoplasmic material inside a singlemembraned vesicle (indicated by an arrow). (C-D) Two examples of large bacteria-containing vesicles, as frequently observed in dram1 overexpressed embryos infected with Mm. V= remnants of vesicular fusion; N = nucleus; « = doublemembrane.