ZFIN Image: Ebert et al., 2014, Fig. S3

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Figure Caption

Fig. S3

Reduced proliferation in sema6a and plxna2 morphant eyes. (A-D) Dorsal views of wholemount control (A,C) and plxna2 morphants (B,D) processed for rx3 mRNA in situ hybridization at 8 (A,B) and 16 (C,D) somites. rx3 is downregulated by 16 somites in a plxna2 morphant, specifically in the eye (arrows). (E-J) Dorsal views of GFP+ eye vesicles immunostained with anti-pHH3 (red) in control (E,F), plxna2 (G,H), and sema6a (I,J) morphant at 20 somites. (K) Quantitation of the numbers of pHH3+ cells within the eye vesicles of control and morphant embryos. N=2. Error bars represent s.e.m (***, P<0.001; One way ANOVA, Dunnett’s test).

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