ZFIN Image: Fuller et al., 2014, Fig. 7

Image

Figure Caption

Fig. 7

Rab23 functions in KV to regulate left-right patterning in zebrafish embryos. (A and B) Expression of fluorescein-tagged rab23-atgMO in the dorsal forerunner cells (DFC) at tailbud stage. Embryos were injected with the MO at midblastula stages to target specifically the DFC that form KV later in development. KV morphology appeared normal in the DFC^rab23MO morphants at the 8-somite stage, as indicated by the arrow in (C). (D) Quantification of spaw expression in the DFC^rab23MO morphants at the 16 to 18-somite stage shows that 40% displayed bilateral spaw expression in the LPM. (E) Quantification of lefty2 expression shows that about half of the DFC^rab23MO expressed lefty2 normally on the left, while the remaining 50% showed bilateral, right-sided, or no lefty2 expression in the heart. Differences in morphant versus control patterns in D and E were highly significant (P<10⁶ Fisherós exact test).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 391(2), Fuller, K., O'connell, J.T., Gordon, J., Mauti, O., Eggenschwiler, J., Rab23 regulates Nodal signaling in vertebrate left-right patterning independently of the Hedgehog pathway, 182-195, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.