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Fig. S7

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Publication: Smith et al., 2013 - A compact, in vivo screen of all 6-mers reveals drivers of tissue-specific expression and guides synthetic regulatory element design
All Figures: Figures for Smith et al., 2013

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Fig. S7 Four positive enhancers that were chosen randomly (in Microsoft Excel) from a list of 35,045 H3K4me1+/me3- hotspots. (a) Random1 is the sole region to produce notochord expression (23/50 embryos at 24 hpf) and also produced considerable somitic muscle expression. (b-d) Three enhancers with forebrain expression. For two of these (Random 2 and 4), GFP expression was strong and specific. The third (Random 3) produced considerable expression in the somitic muscle and spinal cord, with marginal forebrain expression (18/45 embryos at 24 hpf). The complete data set is available in Additional File 7.

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