Fig. 6

Fig. 6

Calcineurin Inhibitors Promote Proximal Growth Program

(A) DMSO-treated fin 28 days after ventral proximal and dorsal distal cuts (red lines indicate growth).

(B) FK506-treated fin 28 days after ventral proximal and dorsal distal cuts (red lines indicate growth).

(C) Early growth rates of DMSO-treated regenerating caudal fin lobes after proximal or distal amputation.

(D) Early growth rates of FK506-treated regenerating caudal fin lobes after proximal or distal amputation.

(E) Regeneration profiles of DMSO-treated fins amputated proximally or distally.

(F) Regeneration profiles of FK506-treated fins amputated proximally or distally.

(G) Adult caudal fin before amputation.

(H) DMSO-treated fin 28 dpa.

(I) Caudal fin before amputation.

(J) FK506-treated fin at 28 dpa. In (G)–(J), red lines show length to ray bifurcation (dashed black line).

(K) Quantification of the measurements of rays from the base to the bifurcation points in (G)–(J).

(L) Graph shows the difference in the shift in bifurcation between DMSO- and FK506-treated caudal fins at 28 dpa.

(M) Whole-mount ISH for shh in distal cut DMSO-treated regenerating fin at 14 dpa.

(N) ISH for shh in proximally amputated fin at 12 dpa.

(O) shh expression domain in distally cut regenerating FK506-treated fins at 12 dpa.

(P) shh domain of during regeneration of FK506-treated proximally cut fin at 12 dpa.

(Q) Length of shh expression domains in proximal and distal amputated DMSO- and FK506-treated fins at 12 dpa.

(R) Length of bmp8a expression domains in proximal and distal amputated DMSO- and FK506-treated fins at 12 dpa.

Scale bars, 2 mm (A and B) and 200 μm (G–J and M–P). Data are represented as mean ± SD from three experiments containing six or more animals (C–F, K, L, Q, and R). p values are results from unpaired t test (K, L, Q, and R).