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Fig. 4
FK506-Enhanced Regenerative Outgrowth Is Not Due to Immunosuppression
(A, D, and G) Caudal fins before treatments and amputation.
(B, E, and H) Corresponding amputated adult fins pretreatment.
(C) DMSO-treated regenerated fin at 35 dpa.
(F) Rapamycin-treated regenerated fin 35 dpa.
(I) FK506-treated regenerated fin at 35 dpa.
(J) Graph of fin-to-body length ratios during the course of regeneration. Dashed line indicates original fin-to-body-length ratio.
(K) Fin-to-body length ratio measurements during the regeneration time course of DMSO- and Celastrol-treated fish.
(L) Fin-to-body-length measurements during regeneration time course from fish injected with DMSO, FK506, rapamycin, or Celastrol.
In (C), (F), and (I), black arrowheads mark the amputation planes. Black lines show the extent of regenerative outgrowth and red lines highlight the length of the entire caudal fin. Scale bars, 1 mm (A–I). In (J)–(L), data are represented as mean ± SD from two or more experiments containing six or more animals.
Reprinted from Developmental Cell, 28, Kujawski, S., Lin, W., Kitte, F., Börmel, M., Fuchs, S., Arulmozhivarman, G., Vogt, S., Theil, D., Zhang, Y., Antos, C.L., Calcineurin regulates coordinated outgrowth of zebrafish regenerating fins, 573-87, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell