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Fig. S5 Zebrafish Kiss1 immunoreactivity in the habenula and the median raphe. Immunohistochemistry using a newly generated antiserum to preprozebrafish Kiss1 showed Kiss1 immunoreactive cells in the ventral habenula (vHb; A) and immunoreactive fibers in the ventral interpeduncular nucleus (vIPN; B) and the median raphe (MR; C) through the fasciculus retroflexus (FR). No further immunoreactive fibers were seen after the MR, suggesting that the habenular Kiss1 neurons are terminated at the MR, but not in the vIPN. (Scale bars, 50 µm.)
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Full text @ Proc. Natl. Acad. Sci. USA