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Fig. S3
Cdh23, Harmonin, Myo7aa and Ift88 localize at the level of the mechanoreceptor. (A-I′) Wild-type zebrafish anterior maculae immunolabeled for Cdh23 (A,B), Harmonin (C,D,I), or Ift88 (E,F). As a control for the binding specificity of the AB Complex, primary antibody was omitted during the reaction (H). As a control for the specificity of the second antibody detection reaction, the first reaction was developed (I), then the AB complex was quenched and blocked (see material and methods), followed by omission of the other primary antibody during the reaction (I′). Both controls resulted in an absence of signal. Confocal sections focused on the hair bundle region. Note the appearance of the hair bundle depends on its spatial distribution along the anterior macula and on the mounting angle during image acquisition (compare panels A-G,I). Yellow arrowheads: hair bundle localization. Arrows: kinociliary localization. Cell bodies in different plans are highlighted in different colors. Scale bar: 5 µm.