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Fig. S6
PSM length in heat-shocked tg+;dlc- embryos is the same as in heat-shocked wildtype. (A) tg+;dlembryos, along with wildtype controls, were taken at the 13 somite stage, given a series of heat-shocks and then stained by double FISH for mespb mRNA (red channel) and deltaC mRNA (green channel). The distance from the posterior tip of the embryo to the middle of the mespb expression domain (asterisk) was measured (yellow line). (B) Graph of length measurements for tg-;dlC+ (n=5) and tg+;dlC- (n=5) embryos after 3 standard heat-shocks (15+25 minutes), fixed 25 minutes after the end of the last heat shock. The values for wildtype and mutant were respectively 364 ± 4 μm and 360 ± 7 μm (mean ± SEM). (C) Graph of length measurements for tg-;dlC+ (n=6) and tg+;dlC- (n=5) embryos after 4 heat-shocks repeated with periodicity 15 + 65 minutes, fixed 65 minutes after the end of the last heat shock. The values for wildtype and mutant were respectively 328 ± 11.6 μm and 335 ± 17 μm (mean ± SEM). The differences in B and C are not statistically significant (two-tailed t-test).