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Fig. S1 Loss of tfap2a and foxd3 individually alters the pattern but does not abolish the expression of scxa. Morpholino- mediated knockdown of tfap2a results in (A-B) reduction of sox9a-positive pharyngeal cartilage and (E-F) an altered pattern of scxa expression at 48 hpf. Morpholino-mediated knockdown of foxd3 results in (C-D) significant loss of sox9a-positive pharyngeal cartilage and (G-H) a significant loss of scxa-positive craniofacial tendon progenitors at 72 hpf. Ventral views of flat-mounted embryos. Controls for Kaede antibody staining for the secondary alone (I) or in non-transgenic embryos (J) did not show any staining when imaged at the same levels as in Figure 4 H-J.