|
Fig. S1 Early developmental expression of nr2f2 in the zebrafish. (A-G) Whole mount in situ hybridization images of developing 10.5 hpf whole lateral (A), 16.5 hpf anterior dorsal (B), 16.5 hpf whole lateral (C), 18 hpf whole lateral (D), 19 hpf head dorsal (E), 19 hpf trunk lateral (F), 24 hpf trunk lateral (G), and 48 hpf trunk lateral (H) views of zebrafish embryos probed for nr2f2. At 3s (A) and 15s (B,C) nr2f2 is expressed in the midbrain and cerebellum (white arrowhead in A) and the spinal cord (white arrowhead in B). This expression persists throughout development (E), but at 18s (D) expression is first identified in the developing axial vessels (black arrowhead). At 20s (F), 24 hpf (G), and 48 hpf (H) this vascular expression is confined to the PCV. The images in panels G and H are the same as those in Fig. 1B and H, respectively. Scale bars, (A,C) 200 µm, (B,DH) 100 µm.
Reprinted from Developmental Biology, 390, Swift, M.R., Pham, V.N., Castranova, D., Bell, K., Poole, R.J., Weinstein, B.M., SoxF factors and Notch regulate nr2f2 gene expression during venous differentiation in zebrafish, 116-25, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.