ZFIN Image: Le Guen et al., 2014, Fig. S6

Image

Figure Caption

Fig. S6

Tg(shh:vegfc) partly rescues lymphatic development in ccbe1^hu3613 mutants but not in ccbe1 morphants. (A) Confocal projections in the Tg(flt4:YFP) background. In wildtype embryos PLs are visible at 3.5dpf (arrows) and TD is present at 5dpf (arrow heads), but not in ccbe1^hu3613 mutants, PLs and TD are missing (asterisks). However upon vegfc overexpression in the Tg(shh:vegfc) some PLs (arrows) and TD fragments (arrow heads) are observed in ccbe1^hu3613 mutants. (B) Quantitation of PLs and TD rescue in the Tg(shh:vegfc) in the absence of ccbe1. When vegfc is ectopically expressed in the floorplate, more than 70% of ccbe1^hu3613 mutants have PLs, and 55% develop TD fragments. (C) Confocal projections in the Tg(flt4:YFP) background. In wildtype embryos PLs are visible at 3.5dpf (arrows), but not in ccbe1 morphants (asterisks). Upon vegfc overexpression in the Tg(shh:vegfc) PLs (asterisks) are not rescued in ccbe1 morphants. (D) Quantitation of PLs rescue in the Tg(shh:vegfc) in wildtypes and ccbe1 morphants. When vegfc is ectopically expressed in the floorplate, 0% of ccbe1 morphants have PLs. (E) In the Tg(hsp70l:Gal4;4xUAS:vegfc) line no PL or TD rescue was observed after ccbe1 morpholino injection.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development