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Fig. S1
The ability of Bad to radiosensitize zebrafish embryonic neural tissue is conserved between zebrafish and human. Shown are lateral views of representative tails from 27-hpf wild-type embryos injected with 50 pg of mRNA encoding mcherry (cntl) or hBAD. Apoptosis in irradiated embryos is denoted with arrowheads. At 24 hpf, half of each group was irradiated with 8 Gy IR and analyzed three hours later by the Casp3 assay. Fluorescence intensity was measured in the spinal cords of at least 10 embryos from each group. Data represent one experiment, but the experiment was independently performed three times with similar results.