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Fig. S5
Cell number and mesenchymal differentiation is not impaired in Rab11DN embryos. (A) Quantification of total cell number in the gut in WT and Rab11aDN embryos. WT n=13, DN n=11, P>0.32. (B,C) Lateral view of an in situ hybridization showing αSMA expression in WT and Rab11aDN embryos at 72 hpf. Arrow points to smooth muscle. Scale bar: 100 μm. (D-E′′) Confocal section of WT and Rab11DN embryo stained for Myh11. Arrowhead points to Mhy11 in the mesenchyme. Asterisk indicates non-specific epithelial staining as observed previously (Wallace et al., 2005). Scale bar: 20 μm.
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