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Fig. S6
Interaction of Wnt/β-catenin signaling with other signaling pathways, related to Figure 6.
(A) Overexpression of the Bmp inhibitor noggin3 (nog3) for 6 hours interferes with lef1 expression in hs:nog3 transgenic regenerates at 3 dpa.
(B) ptc2 expression is detected in the basal epidermal layer and in (pre)osteoblasts (arrowhead).
(C) dkk1 overexpression strongly interferes with regenerative growth which cannot be rescued by concomitant administration of RA via intraperitoneal injections. n = 5 fish, 6 rays each. Error bars indicate error of the mean.
(D) fgf3 (blue) and mCherry (brown) transcripts co-localize in the distal-most blastema (arrowhead) in 7xTCF:mCherry transgenic regenerates. Note that samples were stained for mCherry expression only shortly, so that only the strongest expression domain in the distal blastema was detected.
(A-D) Small arrowheads: amputation plane; scale bars: whole mounts, 200 μm and 100 μm (D); sections, 100 μm.