ZFIN Image: Wehner et al., 2014, Fig. 6

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Figure Caption

Fig. 6

Wnt/β-Catenin Signaling Regulates Blastema Proliferation via Hedgehog and Retinoic Acid Signaling

(A) noggin3 overexpression reduces lef1 (blue) but not mCherry (brown) expression in 7xTCF:mCherry; hs:nog3 double transgenic regenerates.

(B) Overexpression of a dominant negative fgfr1 reduces lef1 (blue) but not mCherry (brown) expression in 7xTCF:mCherry; hs:dnfgfr1 double transgenic regenerates.

(C) axin1 overexpression inhibits expression of the Fgf targets pea3 and erm, which is rescued by concomitant activation of Fgf/Ras signaling via overexpression of v-ras.

(D) axin1 overexpression inhibits expression of the Hh target ptc2, which is rescued by concomitant activation of Hh signaling using the pharmacological compound SAG.

(E) axin1 overexpression reduces the number of H3P⁺ cells in the blastema, which is rescued by concomitant activation of Hh signaling using SAG.

(F) Inhibition of Hh signaling through exposure to cyclopamine reduces the number of H3P⁺ cells in the blastema, which is not rescued by concomitant activation of Wnt signaling following wnt8 overexpression.

(G) Administration of RA via intraperitoneal injection rescues the reduction of H3P⁺ blastemal cell numbers caused by axin1 overexpression.

In (A)–(G), arrowheads indicate amputation plane. Scale bars, 200 μm. Error bars indicate SEM.

Acknowledgments

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