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Fig. S10
The PI3K inhibitor LY294002 affects sub-cellular localization of Foxo3b. (a) GFP-tagged Foxo3b predominately resided in the cytoplasm of HEK293T cells. (b) GFP-Foxo3b translocated to nucleus of HEK293T cells after treatment with LY294002 (PI3K inhibitor). (c) GFP-tagged Foxo3bTM, in which the three conserved, putative Akt phosphorylation sites were mutated to alanine (T30A, S223A, S289A), located primarily in the nucleus of HEK293T cells.
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Reprinted from Developmental Biology, 388(1), Hu, B., Zhang, W., Feng, X., Ji, W., Xie, X., and Xiao, W., Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.