ZFIN Image: Hu et al., 2014, Fig. S3

Image

Figure Caption

Fig. S3

Eaf1-mismatch mRNA can partially rescue the hematopoietic defects seen in eaf1 morphants. (A) O-dianisidine staining of the wildtype control embryos (a, d and e), the embryos co-injected with Eaf1-MO1 and GFP-mRNA (b, f and g), and the embryos co-injected with Eaf1-MO1 and eaf1 mismatch-mRNA (c, h and i) at 52hpf. (B) The reduction of hbbe1(b) in the blood island of eaf1morphants was restored to the level of wildtype embryos by co-injected with eaf1-mismatch mRNA at 48hpf(c). (C) The reduction of l-plastin (b) in eaf1 morphants was partially restored by co-injected with eaf1-mismatch mRNA at 48 hpf(c). (D) The reduction of mpo (b) in eaf1 morphants was partially restored by co-injected with eaf1-mismatch mRNA at 48 hpf (c). (E)Expression of injected Eaf1-mismatch mRMA was confirmed by Western blot assays using anti-Myc antibody. Eaf1-MO1, 8-16ng/embryo; Eaf1-mismatch mRNA, 600pg/embryo.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 388(1), Hu, B., Zhang, W., Feng, X., Ji, W., Xie, X., and Xiao, W., Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.