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Fig. S2
Knockdown of zebrafish eaf1 by morpholinos targeting either ATG (Eaf1-MO1) or 5′-UTR (Eaf1-MO2) blocked hematopoiesis, but not by Eaf1-mismatch-MO. O-dianisidine staining of control and eaf1 depleted-embryos at 36hpf, 52hpf and 3.5dpf. (A) Knockdown of eaf1 by either Eaf1-MO1 or Eaf1-MO2 resulted in reduction of hemoglobin staining at 36hpf (b,c), 52hpf (e, f), 3.5dpf (h, i). (B) Hemoglobin was not detectable in embryos injected with Eaf1-MO1 (c, d) as compared with control (STD-MO) (a, b) at 6.5 dpf. (C) O-dianisidine staining at 36hpf (a-d) and 52hpf (e-h) showed no obvious change for hemoglobin level between the wildtype embryos (control) (a, b, e, f) and the embryos injected with Eaf1-mismatch-MO (c, d, g, h). Eaf1-MO1, targets ATG to block translation; Eaf1-MO2, targets the 5′ un-translated region to block translation; Eaf1-mismatch-MO, a morpholino harbors 5 mismatched nucleotides corresponding to Eaf1-MO3. Eaf1-MO1, 8-16ng/embryo; Eaf1-MO2, 8-16ng/embryo; Eaf1-mismatch-MO, 8-16ng/embryo.
Reprinted from Developmental Biology, 388(1), Hu, B., Zhang, W., Feng, X., Ji, W., Xie, X., and Xiao, W., Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis, 81-93, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.