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Fig. S6
Non-specific MO-induced apoptosis was not observed in p75, TROY and Nogo-C2/NgRH1a morphants. Each MO was injected into the CldnB::lynEGFP transgenic line, and then collected the morphants with malformed PLL primordium at 26–34 hpf for TUNEL assay. One embryo at 30 hpf, representative of a large sample (n = 10), was shown. Apoptotic cells in PLL primordium in control embryos (panels A–A23), and p75 (B–B23), TROY (C–C23), and Nogo-C2/NgRH1a (D–D23) morphants were revealed by TUNEL staining in red. Nuclei were also stained with DAPI. Scale bar, 20 μm.
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