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Fig. S6
(A) Appearance and perdurance of Vangl2 protein induced by heat shock treatment
Embryos obtained by crossing Tg(UAS:Vangl2 FL) F1 fish to Tg(hsp70:Gal4) fish, were heat shocked at 10 hpf, fixed at 12-48 hpf, and processed for anti-Myc immunostaining to identify Vangl2-expressing cells. Myc-positive staining (arrows) was apparent on plasma membranes by 15 hpf (5 hours post heat shock), subsequently increased in levels, and was maintained until 48 hpf. (B) Weaker effects on FBM neuron migration with heat shocked F3 embryos
Summary of effects on FBM neuron migration following heat shock-induced Vangl2 FL transgene expression in F3 embryos. Embryos obtained by crossing Tg(UAS:Vangl2 FL) F2 fish to Tg(hsp70:Gal4) fish, were heat shocked at various indicated times, and migration phenotypes were scored at 48 hpf. Although induction of Vangl2 FL expression at different times interfered with FBM neuron migration, the penetrance of the phenotype was weaker than in F2 embryos (Fig.5). Nevertheless, the strongest effects (i.e., embryos with all FBM neurons stuck in r4) were observed only in embryos heat shocked between 10-15 hpf. Number of embryos scored in parenthesis. Data pooled from two or more experiments.
Reprinted from Mechanisms of Development, 131, Pan, X., Sittaramane, V., Gurung, S., and Chandrasekhar, A., Structural and temporal requirements of Wnt/PCP protein Vangl2 function for convergence and extension movements and facial branchiomotor neuron migration in zebrafish, 1-14, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.