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Fig. 6 Dominant-negative papc inhibits convergence movements of paraxial mesoderm. (A) Control embryo injected with lacZ mRNA. (B) Embryo with widened somites caused by injection of DN-papc mRNA. (C) Embryo injected with DN-papc showing unilateral lack of trunk somites. (D) Embryo injected with FL-papc mRNA (100 pg) and stained with myoD probe; somites and the adaxial muscle cells flanking the notochord are normal. (E) DN-papc mRNA injection causes lateral expansion of myoD expression; axial mesoderm is normal. (F) Reduction of myoD expression caused by DN-papc injection, a few myoD-positive cells are seen in lateral positions but not in the paraxial region. (G) Embryo injected with lacZ mRNA. lacZ is in red and myoD in blue. Somites are normal and lacZ activity is seen on both sides. (H) Widened somites on the injected side of embryo injected with DN-papc mRNA. (I) Embryo that received mRNA bilaterally showing expanded somites on both sides. The amount of mRNA injected was 50 pg for DN-papc and 500 pg for lacZ in all cases.