Disorganized head formation in six3 mRNA-injected zebrafish embryos. 20 pg of globin (A,C,E,G) or six3 mRNA (B,D,F,H) were injected into 2-cell-stage zebrafish embryos; see Fig. 7 for quantitative results. (A,B) Dorsal views at 24 hours. Three ventricles (open triangles) in the brain are filled with masses of cells and the midbrain-hindbrain boundary (arrow) is disorganized in six3 mRNA-injected embryos. (C,D) Dorsal views of Islet-1 expression at 24 hours. The region between AC and epiphysis (bracket) is enlarged in six3 mRNA-injected embryos. Likewise, the distance between left and right trigeminal ganglia is expanded. (E-H) Dorsal (E,F) or lateral views (G,H) of 36 hour embryos labeled with antibody against acetylated α-tubulin. AC (arrow), POC (thin arrow), SOT (black triangle) and region surrounded by them are enlarged, while axons in PC (open arrow) are reduced in six3 mRNA-injected embryos. Additional abbreviations: ot, otic vesicle; tg, trigeminal ganglion. Scale bar, 200 μm.
ZFIN wishes to thank the journal for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if
additional information is required.
Oops. Something went wrong. Please try again later.