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Fig. 5 Effect of fss and yot mutations on the primary motoneurons. (A,B) Znp-1 staining of wild-type sibling and fss mutant, respectively. The number of CaP axons is slightly increased in fss as compared to wild type, and some axons terminate prematurely. (C,D) Znp-1 staining of wild-type sibling and yot mutant, respectively. In C, CaP axons project down in every segment. In D only four CaP axons are projecting down. (E) Same yot mutant as in D; high power magnification of five myotomes anterior to the anus. One CaP axon projects into the muscle. In the other segments axons are running posteriorly, parallel to the neural tube.