ZFIN Image: He et al., 2014, Fig. 8

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Figure Caption

Fig. 8

Vsx1 is a transcriptional repressor. (A) Schematic representation of the Vsx1 mutants. (B) Comparison of flh promoter mediated gfp expression under the regulation of a fusion protein En–Vsx1 and a fusion protein VP16–Vsx1. Results are expressed as mean±SEM, and statistical analyses were done by unpaired t test. ^***P<0.001. (F–H) En–Vsx1 fusion protein functions as a wild type Vsx1. (I–K) VP16–Vsx1 fusion protein functions as a Vsx1 antimorph. The injected reagents are indicated at the right side of the images and riboprobes are indicated at the bottom of each figure. (C, F and I) Dorsal view of phenotypes at 8–10 somite stage with head towards the top. (D, G and J) Dorsal view of embryos with animal pole towards the top. (E, H and K) Animal pole view of the embryos with dorsal towards the right. In situ hybridization of flh is shown at 30–40% epiboly stage.

Acknowledgments

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Reprinted from Developmental Biology, 386(1), He, Y., Xu, X., Zhao, S., Ma, S., Sun, L., Liu, Z., and Luo, C., Maternal control of axial-paraxial mesoderm patterning via direct transcriptional repression in zebrafish, 96-110, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.