|
Fig. 7 Transplanted Nkx2.5-expressing cells express cardiac markers in ectopic locations. (A) Schematic of method used to coinject zebrafish donor embryos at the 1- to 2-cell stage with Nkx2.5 RNA and tetramethylrhodamine dextran as described by Lee et al. (1994). A few cells were transplanted to the animal pole of an unlabelled host. They subsequently populated the brain or trunk. B, C and D are confocal microscope images of the brain region shown in A. (B) Donor cells labeled by tetramethylrhodamine dextran. (C) A subset of cells in the same region are reactive to the atrial-specific antibody, S46. (D) A superimposed image of B and C, showing that all S46 immunoreactive cells are also rhodaminelabeled, and hence derived from the donor.