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Fig. 6 Injection of higher doses of Nkx2.5 mRNA (250 pg) causes ectopic myosin heavy chain expression. In the chest region, this is evident as (A) two beating hearts (arrows), shown in their two pericardial sacs or (B) with MF20 labeling (arrows). Elsewhere, patches of ectopic MF20 immunoreactivity may be found (C,D) in the brain (arrows) or (E,F) on the yolk (arrows). The other MF20 immunoreactive structure is the heart (arrowhead). Embryos are oriented with dorsal to the top and anterior to the left in A,D and F. B is a ventral view and C and E are dorsal views of the embryo. hd, head.
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