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Fig. 4 Isl-1 is expressed in neurons with long axons. (A) Nuclei of the trigeminal ganglion and the anterior part of dorsolateral fascicle (DLF) visualized in a 28 hpf embryo by whole-mount immunostaining with anti-Isl-1 and anti-HNK-1 antibodies; (B) Rohon-Beard nuclei in the spinal cord and the posterior part of dorsolateral fascicle visualized in whole-mount immunostaing using anti-Isl-1 and anti- HNK-1 antibodies (28 hpf); (C,D) whole-mount immunostaining of a 22 hpf embryo using anti-Isl-1 and anti-acetylated a-tubulin antibodies (eyes and yolk were removed before staining). tg, trigeminal ganglion; dr, dorsorostral cluster; vr, ventrorostral cluster; vc, ventrocaudal cluster; ob, olfactory bulb; e, epiphysis; nPC, nucleus of posterior commissure; PC, posterior commissure; DVT, dorsoventral tract; SOP, supra-optic tract; TPOC, tract of postoptic commissure; +, Isl-1-positive cell nuclei in the region where SOT and TPOC fasciculate. Bars, 50 μm.