|
Fig. 4
MITF Regulates Expression of IRF4
(A) The expression of the Mitf and Irf4 mRNAs is reduced in hearts from Mitfmi-vga9 mutant mice, as determined by qPCR analysis. Data are represented as mean ± SEM.
(B) Expression of the MITF, IRF4, TFAP2a, DCT, and TYR genes upon treatment with shRNA against MITF, IRF4, TFAP2A, and negative control in 501mel melanoma cells as determined by qPCR analysis is shown. Data are represented as mean ± SEM.
(C) Western blot shows expression of the MITF, TFAP2A, IRF4, and β-actin proteins in 501mel cells after shRNA treatment. Intensity quantification is relative to actin-loading control. See also Figure S2.
(D) Overexpression of the mouse Mitf (mMitf) cDNA (expressed from pcDNA3.1) in 501mel melanoma cells and in HEK293T embryonic kidney cells affects expression of the TYR and IRF4 mRNAs as assayed by RT-qPCR, whereas β-actin expression is unchanged. mMitf was detected with species-specific primers that only recognize the mouse Mitf gene. See also Figure S3.
See also Figure S2 and Table S3.
Reprinted from Cell, 155(5), Praetorius, C., Grill, C., Stacey, S.N., Metcalf, A.M., Gorkin, D.U., Robinson, K.C., Van Otterloo, E., Kim, R.S., Bergsteinsdottir, K., Ogmundsdottir, M.H., Magnusdottir, E., Mishra, P.J., Davis, S.R., Guo, T., Zaidi, M.R., Helgason, A.S., Sigurdsson, M.I., Meltzer, P.S., Merlino, G., Petit, V., Larue, L., Loftus, S.K., Adams, D.R., Sobhiafshar, U., Emre, N.C., Pavan, W.J., Cornell, R., Smith, A.G., McCallion, A.S., Fisher, D.E., Stefansson, K., Sturm, R.A., and Steingrimsson, E., A Polymorphism in IRF4 Affects Human Pigmentation through a Tyrosinase-Dependent MITF/TFAP2A Pathway, 1022-1033, Copyright (2013) with permission from Elsevier. Full text @ Cell