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Fig. 7 miR-153 is expressed in motor neurons.
To enrich for motor neurons, heads were removed from 52 hpf embryos just posterior to the otic vesicle and trunks were dissociated to facilitate sorting of RFP+ and RFP- cells. RNA was isolated from these cell fractions and RT/PCR was performed to determine miR-153 levels relative to U6 snRNA. Significance was determined by a two-tailed Student’s t-test with the error bars representing s.e.m.; p<0.02.
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