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Fig. S2

ID
ZDB-IMAGE-140124-2
Source
Figures for Matsuda et al., 2013
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Figure Caption

Fig. S2 Aldh1-expressing cells and Notch-responsive cells represent distinct cell types in adult zebrafish pancreas. (A-D) FACS sorting was performed on single cells isolated from adult pancreas of wild type (A and B) or Tp1:eGFP (C and D) fish. Wild-type cells were labeled with Aldefluor and then FACS sorted, while cells from Tp1:eGFP fish were sorted for eGFP. Sorted populations were then subjected to cytospin and immunofluorescent labeling for Aldh1. In the case of cells from Tp1:eGFP fish, labeling was also performed for eGFP. Note that Aldh1 protein is detected in Aldefluorpos but not Tp1:eGFPpos cells, while eGFP is detected in Tp1:eGFPpos but not Aldefluorpos cells. Arrows in (C) indicate low-abundance Aldh1-sorted cells present in Tp1:eGFPneg but not Tp1:eGFPpos cell fraction. E, RT-PCR analysis of gene expression in FACS sorting populations. Positive and negative populations of cells sorted for either Aldh1 activity or Tp1:eGFP expression displayed differential expression of aldh1a2, prom1, and sca1, with contrasting patterns of enrichment in Aldefluorpos vs. Aldefluorneg and Tp1:eGFPpos vs. Tp1:eGFPneg cell fractions, further documenting the non-overlapping nature of Aldh1-expressing and Notch-responsive progenitor cells. Note that both populations are depleted of transcripts encoding differentiated endocrine (insulin), acinar (elastase1) and ductal (krt18) markers.

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