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Fig. S6
Inactivity reduces myofibrilogenesis more significantly than rapamycin. Zebrafish embryos were treated with MS222, rapamycin, both, or vehicle control from 31–32 hpf and analyzed at 48 hpf. (A) Immunofluorescence of slow MyHC on whole-mount embryos treated with either MS222 or rapamycin for 17 h. Note that brightness was enhanced on MS222 image to show myofibril bundles. (B) Effect of rapamycin on pS6 immunoreactivity in muscle. (C) Quantification of slow fiber myofibril bundle width. Mean ± SEM (n = 8 embryos in each condition, 10 fibers measured per embryo). (D) Western analysis of Tropomyosin, Actin, and Fast MyLC in the presence of MS222, rapamycin, or both.