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Fig. 5
Par3 is required for CIL in vivo in zebrafish. Single frames from time-lapse movies illustrating a collision between two cranial NC cells (1 and 2) in a zebrafish embryo injected with ControlMO (A,B) or Par3MO (D,E). Images are overlapped with tracks of migratory paths (A,D) or the difference in protrusion between current and previous frames (B,E). Red area indicates new protrusions. (C,F) Velocity vectors of colliding NC cells in ControlMO-injected (C) or Par3MO-injected (F) embryos. Cell position was recorded at 5-minute intervals. Velocity change was clustered in ControlMO-injected cells (n=10; P<0.05), but no difference in the velocity change was observed in Par3MO-injected cells (n=9; P>0.1). Red arrow indicates the initial velocity vector.