Hyperbranching and venous sprouting is dependent on proper Flt4 signaling. S2A Lateral images and quantification of Tg(fli1a:gfp;flt1enh:rfp) embryos treated as indicated and imaged at 52 hpf. S2C Lateral images and quantification of Tg(fli1a:gfp;flt1enh:rfp) embryos treated as indicated and imaged at 52 hpf. S2D, S2E, S2F, quantification of the indicated parameters. Concentrations: dll4 MO (low 1.5 ng in S2A and 3 ng in S2C–F); e2f7/8 mRNA (100 pg each); flt4 mRNA (100 pg); ccbe1 mRNA (100 pg). Arrows depict PLs that have connected to ISVs (S2C). Closed arrow heads indicate (upper panel in S2C) PLs or (lower panel, S2C) presence of the TD. Open arrowheads indicate hyperbranching ISVs. All scale bars are 100 μm. Stars indicate missing TD fragments. Data presented as the average (±s.e.m.) compared to the control condition in three independent experiments (*** P<0.001). At least n = 150 embryos per condition in three independent experiments were used for S2A–S2F.
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