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Fig. S4
Body axis elongation defects are induced by alternative ppp1cba and ppp1cbb morpholinos but not mismatched controls. (A–C) Lateral views of representative 48 hpf zebrafish embryos injected with (A) 2.5 ng mismatch ppp1cba MO, (B) 2.5 ng mismatch ppp1cbb MO, (C) a mixture of 0.75 ng mismatch ppp1cba MO and 0.75 ng of mismatch ppp1cbb MO (2MO-MM), (D) 5.0 ng ppp1cba MO-2, (E) 5.0 ng ppp1cbb MO-2, (F) a mixture of 1.5 ng ppp1cba MO and 1.5 ng of ppp1cbb MO (2MO-2). (G) Quantification of the truncated body axis phenotype in morphant and control embryos. Each injection was performed multiple times with 25 embryos used to calculate body axis length and reported as % of uninjected clutch mates. Error bars are standard error and a black * indicates a statistically significant difference from control. Statistical significance was calculated using a one-factor ANOVA with Tukey post hoc analysis and is defined as p < 0.05.