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Fig. 4 Manipulation of Eph/Ephrin activity leads to defective segregation of eye and telencephalic cells. Frontal views of the forebrain and eyes in Tg{rx3:GFP} (A-C) and Tg{emx3:YFP} (D-F) 10-12 ss wild-type, morphant or mRNA-injected zebrafish embryos immunostained to detect GFP (green), ZO-1 (blue) and F-actin (red). Arrows point at eye fated cells located in the telencephalic domain (B,C) or at telencephalic fated cells located in the optic vesicles (E,F). The dotted lines demarcate the transition between telencephalon and eye. ov, optic vesicle; tel, telencephalon; hyp, hypothalamus. Scale bars: 50 μm.
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