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Fig. S4

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ZDB-IMAGE-131218-15
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Figures for Sittaramane et al., 2013
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Figure Caption

Fig. S4 Mesodermal vangl2 expression is not required for FBM neuron migration.
(A) Targeting of TAR RNA-injected donor cells to the endoderm and mesoderm in Tg(isl1:GFP) hosts. Hindbrain cross section at the r4 level in an 18 hpf host embryo stained for host FBM neurons (GFP antibody, white arrowheads) and donor-derived cells in the endoderm (e) and mesoderm (m), including the notochord (n) (staining of biotin-dextran in donor cells, black arrowheads). (B,C) Dorsal views of Tg(isl1:GFP) host hindbrain (B), with the level of r4 crosssection (C) indicated by the broken line. Targeting of vangl2 morphant cells to the mesoderm (black arrowheads, C) in a wildtype host (B) does not have a significant effect on the migration of host neurons (white arrowheads in B, C). oto, otic vesicle.

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Reprinted from Developmental Biology, 382(2), Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A., The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function, 400-412, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.