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Fig. 4 glypican4 and frizzled7 mutant embryos have increased fibronectin assembly. (A–C) Top panels show morphological convergence and extension phenotypes at tailbud stage (see arrowheads) of wild type, glypican4 mutant, and MZfrizzled7a/7b mutant embryos. Middle two panels show confocal images of fibronectin (Fn) immunolabeling without and with nuclear DAPI staining. Scale bars=20 µm. The bottom images in (A) and (B) show Fn expression in cross-sections (x.s.) of tailbud stage embryos (n, notochord).
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Reprinted from Developmental Biology, 383(1), Dohn, M.R., Mundell, N.A., Sawyer, L.M., Dunlap, J.A., and Jessen, J.R., Planar cell polarity proteins differentially regulate extracellular matrix organization and assembly during zebrafish gastrulation, 39-51, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.