Fig. 2

Fig. 2 Prickle1a binds Vangl2 and regulates its expression. (A) Western blot (WB) analysis after Vangl2 antibody immunoprecipitation of transfected CHO cell protein extracts. Incubation of protein extracts with Vangl2 antibody co-immunoprecipitates both Vangl2 and Myc-Prickle1a. Whole cell lysates (WCL) demonstrate successful transfection and expression of specific proteins. (B) Confocal images of Vangl2 expression in tailbud stage wild type and prickle1a morphant embryos. Scale bars=20 μm. (C) Quantification of membrane versus cytoplasmic Vangl2 expression; 465 wild type control measurements from three embryos; 742 prickle1a morphant measurements from four embryos (*p<0.0001). (D) Western blot analysis of Vangl2 protein expression normalized to GAPDH in wild type and prickle1a morphant embryos. Bar graph represents data from three independent experiments and the blot depicts results from one of those experiments (*p<0.007).