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Fig. S3 Epithelial development and polarity is not affected in osr2 morphants. A: Histological section of a control embryo at the level of the mid-trunk shows cross-sections of the kidney (arrowheads) and gut (arrow) stained with anti-ZO-1, highlighting polarized apical cell–cell junctions. Both osr2 ATG morphants (B) and osr2 exon3 splice morphants (C) also show normal epithelial polarized junctions in the kidney and gut. Blue, DAPI stained nuclei. Representative sections from n=3 for each morpholino.
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