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Figure Caption
Fig. S2 scl-β:d2eGFP+ endothelial cells give rise to scl-β:d2eGFP+/scl-α:DsRed+ HSCs. Time-lapse confocal imaging of a live Tg(scl-β:d2eGFP; scl-α:DsRed) embryo between 34 and 40 hpf. Four selected time points show the stepwise transition of an scl- β:d2eGFP+ endothelial cell to an scl-β:d2eGFP+/scl-α:DsRed+ HSC via EHT (blue arrows). The intensity of DsRed signal is increased as the cell bends outwards. For each time point, d2eGFP, DsRed and merged images are presented. White arrow indicates the direction of circulation in DA. DA, dorsal aorta; AV, axial vein. Scale bar: 20 μm.
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