ZFIN Image: Choorapoikayil et al., 2013, Fig. S4

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Fig. S4

Rescue of morphological defects in ptena-/-ptenb-/- mutants by exogenous Ptena, LY294002 and Sunitinib. At 4 dpf ptena-/-ptenb-/- mutants display severe morphological defects compared to wildtype (a, b): head and eye edema, wider set eyes, enlargement of yolk sac, reduced body length. Expression of exogenous Ptena did not cause malformation in development of wildtype embryo, but largely rescued the developmental defects in ptena-/-ptenb-/- mutants (c, d). Treatment of embryos from 72 hpf onwards with 25 μM LY294002 resulted in mild growth defects in the head region in wildtype embryos and almost complete rescue of developmental defects in ptena-/-ptenb-/- mutants (e,f). Treatment with 20 μM Sunitinib from 72 hpf onwards did not induce obvious morphological defects in wildtype embryos and largely rescued the morphological malformations in ptena-/-ptenb-/- mutants (g,h).

Acknowledgments

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