ZFIN Image: Choorapoikayil et al., 2013, Fig. 5

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Figure Caption

Fig. 5

Combined treatment with LY294002 and sunitinib rescued hypervascularization. (A) Quantitative PCR was performed to determine vegfaa expression at 4 dpf in ptena^-/-ptenb^-/- mutants compared with siblings following treatment with suboptimal concentrations of LY294002 (LY, 5 μM) or sunitinib (5 μM), or both, from 72 hpf onwards. Three embryos per condition were pooled and used for quantitative PCR; at least two independent experiments were performed. Relative amounts were determined with wild type untreated set to 1.0. Statistical analysis (Kruskal-Wallis with Dunn’s post-hoc test) was performed using Excel and SPSS 20 (IBM); significance is indicated: **P<0.01; ***P<0.001. (B–I) Vasculature of wild-type and ptena^-/-ptenb^-/- embryos at 4 dpf was imaged in the Tg(kdrl:eGFP) line by confocal microscope with 20×. The embryos were treated with suboptimal concentrations of LY294002 (LY), sunitinib or both as indicated. Representative embryos are depicted; anterior to the left.

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Acknowledgments

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