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Fig. S3
Developmental patterning transcription factor expression domains in adult zebrafish fins. (A) Expression of the tbx18:EGFP fluorescent transgenic reporter across the entire AP axis of an adult pectoral fin. (B) Relatively uniform expression of tbx18 is verified by qPCR. Expression is normalized to actb1. n=3. (C) Expression of alx4a:DsRed2, hand2:EGFP and tbx18:EGFP in the indicated adult fin types. Arrows or brackets indicate expression domains. (D) Endogenous transcription factor gene expression is consistent with BAC reporter fluorescence patterns. (Left) RT-PCR supports region-specific expression of alx4a and hand2 in uninjured paired fins. (Right) qPCR indicates decreased expression of hand2 and tbx18 in all uninjured medial fins when compared with paired fins. Expression is normalized to actb1, relative to levels in posterior regions of pelvic fin. n=2.