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Fig. 4
hand2 overexpression exerts its effects during later stages of regeneration. (A,B) No difference in the appearance of hsp70l:hand2 and wild-type clutchmate fins at 3 dpa after a series of daily heat shocks. n=16. (C,D) Blastemal proliferation as measured by BrdU incorporation is also unaffected by hand2 overexpression. n=13. (E) Stunted regeneration in an hsp70l:hand2 pectoral fin compared with a wild-type clutchmate fin at 7 dpa after a series of daily heat shocks beginning at 3 dpa. Representative regenerative growth of wild-type ray 3 is denoted by a dotted line. (F,G) Significant reductions in anterior ray lengths and widths manifest when hand2 is overexpressed during later stages of regeneration. n=8. *P<0.05, Student’s t-test; bar indicates mean. Arrowheads (A,C,E) indicate amputation plane.