Fig. 3
Neurotransmitter properties of neurons derived from dorsal progenitor domains. All images were taken from fish that were 3.5 dpf. All images are cross-sections of the spinal cord. Transgenic fish genotypes are shown at the top and left. Arrowheads and arrows show cells that are positive for both GFP and RFP. (A-C) atoh1a:GFP neurons are positive for vglut2a:RFP. (D-F) gsx2:GFP neurons are positive for vglut2a:RFP. (G-I) All three types of neurons (vglut2a:RFP, glyt2:RFP and gad1b:RFP) are present among gsx1:GFP neurons. (J-L) A GFP neuron driven by dmrt3a:Gal4 is positive for glyt2:RFP. (M) Summary of the neurotransmitter properties of neurons produced by dorsal progenitors. Scale bar: 10 μm.