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Fig. S3

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ZDB-IMAGE-131031-7
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Figures for Suzuki et al., 2013
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Fig. S3

The majority of horizontal cells (HCs) are derived from trβ2:FP-positive cell lineage. (A) Confocal image of the retina of a double-transgenic line Tg (ptf1a:GFP; trβ2:tdTomato) at 54 hpf. HC precursors and HCs were identified by expression of GFP. Fluorescence driven by the exogenous trβ2 promoter is relatively weak in HC precursors undergoing cell division (arrowheads). Open arrowhead marks a mitotic cell without detectable trβ2:tdTomato signal. (B) Ectopic trβ2 in HC precursors and HCs by injecting Tg(UAS:MYFP-2A-trβ2) plasmid into one-cell stage fertilized eggs of Tg(ptf1a:Gal4-VP16) fish (12). At 3 dpf, HCs and amacrine cells were labeled by MYFP expression in the retinas and the morphology of MYFP-expressing HCs appeared normal. Therefore, developmental down-regulation of trβ2 in HC precursors and HCs is not essential for HC differentiation.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA
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