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Publication: Jao et al., 2013 - Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system
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Fig. 3

Biallelic disruption of tyrosinase (tyr) by Cas9 generates mosaic pigmentation phenotypes. tyr gRNA (30 pg) and nls-zCas9-nls RNA (150 pg) were injected into wild-type embryos. (A–E) Lateral views of wild-type (A) and tyr-targeted embryos (B–E) at 2 dpf. tyr-targeted embryos showed different degrees of hypopigmentation, some of them were almost unpigmented (e.g., E). (F–K) Dorsal view of the wild-type (F and I) and tyr-targeted larvae (G, H, J, and K) at 7 dpf (F–H) and 14 dpf (I–K). Mosaic pigmentation defects in tyr-targeted larvae persisted to later stages. (L) T7EI assays showed high mutagenesis rates at the tyr target (93–97%) in six randomly selected tyr-targeted embryos. (Scale bars: 500 μm.)

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