IMAGE

Fig. 4

ID
ZDB-IMAGE-131029-7
Antibodies
Source
Figures for Neugebauer et al., 2013
Image
Figure Caption

Fig. 4

3-OST-6 cell-autonomously controls KV cilia motility and LR patterning, but not cilia length. (A) 3-OST-6 morphant at 48 hpf. Except for heart and gut anomalies, 3-OST-6 morphants were indistinguishable from WT embryos at the gross level. (B) Percentages of reversed heart and gut looping in 3-OST-6 morphants (MO1: n=172, **P<0.001; MO2: n=89, **P<0.001) were increased compared with uninjected embryos (n=411). Co-injection of 3-OST-6 MO1 and MO-resistant 3-OST-6ΔTM ‘rescue’ mRNA resulted in a significant rescue of heart and gut orientation (n=184; **P<0.001). Overexpression of 3-OST-6ΔTM mRNA alone did not alter normal heart and gut looping (n=140). Error bars represent s.d. (C) Percentages of left-sided, right-sided, absent, and bilateral spaw in 3-OST-6 morphants (MO1, n=159; MO2, n=165; uninjected, n=220). DFC3-OST-6 MO1 embryos (n=150) showed a high level of bilateral spaw expression, statistically distinct (**P<0.001) from controls: DFCControl MO (n=145), Yolk3-OST-6 MO1 (n=79), and YolkControl MO (n=90). (D) 3-OST-6 morphants had normal KV cell differentiation (red) and normal cilia length (green) that was comparable to control embryos (see Fig. 2A). (E) 3-OST-6 MO (920 cilia, 18 embryos, 8 SS) had cilia lengths comparable to uninjected embryos (P<0.85). Error bars represent s.e.m. (F) 3-OST-6 MO1 morphants lacked directed movement of beads injected into KV. Each color represents a single tracked bead. (G) Average velocity of fluorescent beads injected into KV. Flow velocity was significantly decreased in 3-OST-6 MO1 (five embryos, five beads/embryo; **P<0.001) compared with controls (see Fig. 2D; supplementary material Movies 1 and 2). Error bars represent s.d. (H) Radius of bead movement assay. Concentric circles were placed at bead start. Beads were scored as exiting the inner circle with a radius of 12.5 µm and/or outer circle with a radius of 25 µm. Each color represents a single tracked bead. (I) Quantification of the radius of bead movement.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development